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验证数据展示

  • WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    WB analysis using 85320-1-RR (same clone as 85320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

    IHC staining of human liver cancer using 85320-1-RR (same clone as 85320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85320-1-RR (CYP4F2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85320-1-PBS in a different storage buffer formulation.

CYP4F2-Specific Recombinant monoclonal antibody, PBS Only

CYP4F2-Specific Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85320-1-PBS

产品说明书

CloneNo. 242307B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85320-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily F, polypeptide 2
别名 CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, 242307B10, Arachidonic acid omega-hydroxylase
计算分子量 60 kDa
观测分子量 50-53 kDa
GenBank蛋白编号NM_001082
基因名称 CYP4F2
Gene ID (NCBI) 8529
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78329
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548).

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