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验证数据展示

  • WB analysis of mouse lung using 21579-1-AP

    WB analysis of mouse lung using 21579-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP2F1 Polyclonal antibody

CYP2F1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21579-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21579-1-AP targets CYP2F1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16124

Product name: Recombinant human CYP2F1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-302 aa of BC109056

Sequence: IEERILEEGSFLLAELRKTEGEPFDPTFVLSRSVSNIICSVLFGSRFDYDDERLLTIIRLINDNFQIMSSPWGELYDIFPSLLDWVPGPHQRIFQNFKCLRDLIAHSVHDHQASLDPRSPRDFIQCFLTKMAEEKEDPLSHFHMDTLLMTTHNLLFGGTKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily F, polypeptide 1
别名 C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1
计算分子量 491 aa, 56 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC109056
基因名称 CYP2F1
Gene ID (NCBI) 1572
RRIDAB_10733220
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP2F1 antibody 21579-1-APDownload protocol
IHC protocol for CYP2F1 antibody 21579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols