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验证数据展示

  • WB analysis of pig liver using 67263-1-Ig (same clone as 67263-1-PBS)

    WB analysis of pig liver using 67263-1-Ig (same clone as 67263-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP2E1 antibody included
  • IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    IHC staining of human liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP2E1 antibody included
  • IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CYP2E1-Specific antibody (67263-1-Ig, Clone: 1F12G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    IF Staining of mouse liver using 67263-1-Ig (same clone as 67263-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 67263-1-Ig (CYP2E1-Specific antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67263-1-PBS in a different storage buffer formulation.

CYP2E1-Specific Monoclonal antibody, PBS Only

CYP2E1-Specific Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 67263-1-PBS

产品说明书

CloneNo. 1F12G7

宿主/亚型

Mouse / IgG2b

种属反应性

mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, Indirect ELISA

别名

CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP2E1 Ready-To-Use IHC Kit. CYP2E1 primary antibody included.

产品信息

67263-1-PBS targets CYP2E1-Specific in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 mouse, rat, pig
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily E, polypeptide 1
别名 CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号NM_000773
基因名称 CYP2E1
Gene ID (NCBI) 1571
RRIDAB_2882534
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05181
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

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