验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21555-1-AP targets CYP26B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15368 Product name: Recombinant human CYP26B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 104-300 aa of BC069443 Sequence: KILMGEHHLVSTEWPRSTRMLLGPNTVSNSIGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRRGIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIFAAYAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 26, subfamily B, polypeptide 1 |
| 别名 | Cytochrome P450RAI-2, Cytochrome P450RAI 2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450 26B1, Cytochrome P450 26A2 |
| 计算分子量 | 512 aa, 58 kDa |
| 观测分子量 | 49-58 kDa |
| GenBank蛋白编号 | BC069443 |
| 基因名称 | CYP26B1 |
| Gene ID (NCBI) | 56603 |
| RRID | AB_10733471 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR63 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| IHC protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| IP protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| WB protocol for CYP26B1 antibody 21555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.
| ||
Brain Struct Funct Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1. | ||
J Neurosci The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map. | ||
J Neurosci Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia. | ||
Front Mol Neurosci Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction. | ||
