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验证数据展示

  • WB analysis of HEK-293 using 16702-1-AP

    WB analysis of HEK-293 using 16702-1-AP

    WB result of CYP20A1 antibody (16702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP20A1 transfected HEK-293 cells.

  • WB analysis using 16702-1-AP

    WB analysis using 16702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16702-1-AP

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16702-1-AP

    IHC staining of human liver cancer using 16702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of MCF-7 using 16702-1-AP

    IP experiment of MCF-7 using 16702-1-AP

    IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

  • Featured Product
  • KD/KO验证

CYP20A1 Polyclonal antibody

CYP20A1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16702-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CYP M, CYP20A1, Cytochrome P450 20A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CYP20A1 fusion protein Ag10099 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 20, subfamily A, polypeptide 1
别名 CYP M, CYP20A1, Cytochrome P450 20A1
计算分子量 462 aa, 52 kDa
观测分子量 45-52 kDa
GenBank蛋白编号BC020616
基因名称 CYP20A1
Gene ID (NCBI) 57404
RRIDAB_2261395
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UW02
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP20A1 antibody 16702-1-APDownload protocol
IHC protocol for CYP20A1 antibody 16702-1-APDownload protocol
IP protocol for CYP20A1 antibody 16702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols