CYP1A2-Specific Recombinant antibody

CYP1A2-Specific Recombinant Antibody for IHC, ELISA
Cat No. 84751-1-RR

产品说明书

CloneNo. 241922C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

CYP1A2, CYPIA2, CP1A2, CP12, Cholesterol 25-hydroxylase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84751-1-RR targets CYP1A2-Specific in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 1, subfamily A, polypeptide 2
别名 CYP1A2, CYPIA2, CP1A2, CP12, Cholesterol 25-hydroxylase
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号NM_000761
基因名称 CYP1A2
Gene ID (NCBI) 1544
RRIDAB_3672134
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05177
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

实验方案

Product Specific Protocols
IHC protocol for CYP1A2-Specific antibody 84751-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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