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验证数据展示

  • WB analysis of mouse liver using 19936-1-AP

    WB analysis of mouse liver using 19936-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 19936-1-AP

    FC experiment of HepG2 using 19936-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CYP1A2-Specific Polyclonal antibody (19936-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of mouse liver using 19936-1-AP

    IP experiment of mouse liver using 19936-1-AP

    IP result of anti-CYP1A2-Specific (IP:19936-1-AP, 4ug; Detection:19936-1-AP 1:300) with mouse liver tissue lysate 4000ug.

  • IHC staining of human liver using 19936-1-AP

    IHC staining of human liver using 19936-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19936-1-AP (CYP1A2-Specific Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CYP1A2 antibody included
  • IHC staining of mouse liver using 19936-1-AP

    IHC staining of mouse liver using 19936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP1A2 antibody included
  • IHC staining of human kidney using 19936-1-AP

    IHC staining of human kidney using 19936-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP1A2 antibody included
  • IHC staining of rat kidney using 19936-1-AP

    IHC staining of rat kidney using 19936-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP1A2 antibody included
  • IHC staining of rat liver using 19936-1-AP

    IHC staining of rat liver using 19936-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP1A2 antibody included
  • IF Staining of HeLa using 19936-1-AP

    IF Staining of HeLa using 19936-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP1A2-Specific antibody (19936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CYP1A2-Specific Polyclonal antibody

CYP1A2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 19936-1-AP

产品说明书

引用文献(63)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

CYP1A2, Cholesterol 25-hydroxylase, CP12, CP1A2, CYPIA2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, rat liver tissue, human kidney tissue, rat kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® CYP1A2 Ready-To-Use IHC Kit. CYP1A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19936-1-AP targets CYP1A2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 1, subfamily A, polypeptide 2
别名 CYP1A2, Cholesterol 25-hydroxylase, CP12, CP1A2, CYPIA2
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号NM_000761
基因名称 CYP1A2
Gene ID (NCBI) 1544
RRIDAB_10638909
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05177
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

实验方案

Product Specific Protocols
FC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IF protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IHC protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
IP protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
WB protocol for CYP1A2-Specific antibody 19936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.

Authors - Qing Zhu
View Article
mouseWB

Cell Host Microbe

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Authors - Yunong Zeng
View Article
humanWB

Cell Host Microbe

Cohabitation facilitates microbiome shifts that promote isoflavone transformation to ameliorate liver injury

Authors - Yunong Zeng
View Article
mouseWB

Nat Commun

Hepatic SNHG9 links gut microbiota to liver protection in drug-induced liver injury.

Authors - Wu Bao
View Article
mouseIF

Hepatology

Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.

Authors - Linjie Lv
View Article
mouseWB

J Adv Res

Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug

Authors - Jing Guo
View Article
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