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验证数据展示

  • WB analysis using 85690-5-RR

    WB analysis using 85690-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85690-5-RR (CYP17A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 85690-5-RR

    IHC staining of mouse testis using 85690-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • IHC staining of mouse testis using 85690-5-RR

    IHC staining of mouse testis using 85690-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • Affinity and Kinetic Characterization of 85690-5-RR

    Affinity and Kinetic Characterization of 85690-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85690-5-RR against Human CYP17A1 were performed. The affinity constant is below 1 pM.

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CYP17A1 Recombinant monoclonal antibody

CYP17A1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85690-5-RR

产品说明书

CloneNo. 243076D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CYP17A1 Ready-To-Use IHC Kit. CYP17A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85690-5-RR targets CYP17A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5899

Product name: Recombinant human CYP17A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-508 aa of BC062997

Sequence: HNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNGNAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 17, subfamily A, polypeptide 1
别名 17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC062997
基因名称 CYP17A1
Gene ID (NCBI) 1586
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450 17A1 (CYP17A1) is a critical steroidogenic enzyme, essential for producing glucocorticoids and sex hormones (PMID: 33781841). The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID: 19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme (PMID: 15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID: 27505042).

实验方案

Product Specific Protocols
WB protocol for CYP17A1 antibody 85690-5-RRDownload protocol
IHC protocol for CYP17A1 antibody 85690-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols