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验证数据展示

  • WB analysis using 85690-5-RR (same clone as 85690-5-PBS)

    WB analysis using 85690-5-RR (same clone as 85690-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85690-5-RR (CYP17A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • Affinity and Kinetic Characterization of 85690-5-RR

    Affinity and Kinetic Characterization of 85690-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85690-5-RR against Human CYP17A1 were performed. The affinity constant is below 1 pM.

CYP17A1 Recombinant monoclonal antibody, PBS Only

CYP17A1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85690-5-PBS

产品说明书

CloneNo. 243076D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP17A1 Ready-To-Use IHC Kit. CYP17A1 primary antibody included.

产品信息

85690-5-PBS targets CYP17A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5899

Product name: Recombinant human CYP17A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-508 aa of BC062997

Sequence: HNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNGNAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 17, subfamily A, polypeptide 1
别名 17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC062997
基因名称 CYP17A1
Gene ID (NCBI) 1586
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05093
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome P450 17A1 (CYP17A1) is a critical steroidogenic enzyme, essential for producing glucocorticoids and sex hormones (PMID: 33781841). The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID: 19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme (PMID: 15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID: 27505042).

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