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Host
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Species Reactivity
0
Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85690-5-RR (same clone as 85690-5-PBS)

    WB analysis using 85690-5-RR (same clone as 85690-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85690-5-RR (CYP17A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    IHC staining of mouse testis using 85690-5-RR (same clone as 85690-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85690-5-RR (CYP17A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85690-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP17A1 antibody included
  • Affinity and Kinetic Characterization of 85690-5-RR

    Affinity and Kinetic Characterization of 85690-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85690-5-RR against Human CYP17A1 were performed. The affinity constant is below 1 pM.

CYP17A1 Recombinant antibody, PBS Only

CYP17A1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85690-5-PBS

产品说明书

CloneNo. 243076D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP17A1 Ready-To-Use IHC Kit. CYP17A1 primary antibody included.

产品信息

85690-5-PBS targets CYP17A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CYP17A1 fusion protein Ag5899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 17, subfamily A, polypeptide 1
别名 17-alpha-hydroxyprogesterone aldolase, CPT7, CYP17, CYPXVII, Cytochrome P450 17A1
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC062997
基因名称 CYP17A1
Gene ID (NCBI) 1586
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05093
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human cytochrome P450c17 enzyme is encoded by the CYP17A1 gene. It is the qualitative regulator of steroidogenesis in the human adrenals.In its absence, aldosterone is produced in the human adrenal zona glomerulosa .The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID;19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme(PMID:15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID 27505042, PMID 27505042).