验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83930-1-RR targets CYP11B2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 11, subfamily B, polypeptide 2 |
| 别名 | 240811E7, ALDOS, Aldosterone-synthesizing enzyme, Corticosterone 18-monooxygenase, CYP11B2, CPN2 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58-60 kDa |
| GenBank蛋白编号 | NM_000498 |
| 基因名称 | CYP11B2 |
| Gene ID (NCBI) | 1585 |
| RRID | AB_3671511 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P19099 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP11B2 antibody 83930-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |