验证数据展示

WB analysis using 10993-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10993-1-AP (Cytochrome c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10993-1-AP
WB result of Cytochrome c antibody (10993-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
WB analysis of HEK-293 using 10993-1-AP
WB result of Cytochrome c antibody (10993-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.
IHC staining of human liver using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CYCS antibody included
FC experiment of HepG2 using 10993-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cytochrome c (10993-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of mouse eye using 10993-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Cytochrome c antibody (10993-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CYCS antibody included
IHC staining of human colon cancer using 10993-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10993-1-AP (Cytochrome c antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CYCS antibody included

Featured Product
KD/KO验证

Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 10993-1-AP

产品说明书

引用文献(629)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (2)

别名

CYC, CYCS

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (629)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, HEK-293 cells, HepG2 cells, C2C12 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected in	human liver tissue, human breast cancer tissue, human colon cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue
Positive FC (Intra) detected in	HepG2 cells

This antibody is not recommended for immunocytofluorescent assays.

Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 583 publications below
IHC	See 33 publications below
IF	See 38 publications below
IP	See 1 publications below
FC	See 1 publications below

产品信息

10993-1-AP targets Cytochrome c in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, monkey, chicken, hamster, sheep, goat, hippospongia
免疫原	CatNo: Ag1455 Product name: Recombinant human Cytochrome c protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-105 aa of BC009578 Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cytochrome c, somatic
别名	CYC, CYCS
计算分子量	12 kDa
观测分子量	12-15 kDa
GenBank蛋白编号	BC009578
基因名称	Cytochrome c
Gene ID (NCBI)	54205
RRID	AB_2090467
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P99999
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
IF protocol for Cytochrome c antibody 10993-1-AP	Download protocol
IHC protocol for Cytochrome c antibody 10993-1-AP	Download protocol
WB protocol for Cytochrome c antibody 10993-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Authors - Tara E Tracy View Article
human	WB,IF	Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration Authors - Jianxiong Chen View Article
human	WB	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang View Article
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
human,rat	WB	Adv Sci (Weinh) Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury Authors - Qiaohui Chen View Article
mouse	WB	Acta Pharm Sin B Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis Authors - Yujun Zhou View Article

验证数据展示

WB analysis using 10993-1-AP

WB analysis of HepG2 using 10993-1-AP

WB analysis of HEK-293 using 10993-1-AP

IHC staining of human liver using 10993-1-AP

FC experiment of HepG2 using 10993-1-AP

IF Staining of mouse eye using 10993-1-AP

IHC staining of mouse brain using 10993-1-AP

IHC staining of human colon cancer using 10993-1-AP

Cytochrome c Polyclonal antibody

Cytochrome c Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

经过测试的应用

This antibody is not recommended for immunocytofluorescent assays.

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis