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验证数据展示

  • WB analysis of human placenta using 11807-1-AP

    WB analysis of human placenta using 11807-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11807-1-AP

    IHC staining of human breast cancer using 11807-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11807-1-AP (CYB5R1 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of human placenta using 11807-1-AP

    IP experiment of human placenta using 11807-1-AP

    IP result of anti-CYB5R1 (IP:11807-1-AP, 4ug; Detection:11807-1-AP 1:1000) with human placenta tissue lysate 1520ug.

  • IF Staining of PC-3 using 11807-1-AP

    IF Staining of PC-3 using 11807-1-AP

    Immunofluorescent analysis of PC-3 cells using 11807-1-AP (CYB5R1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CYB5R1 Polyclonal antibody

CYB5R1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11807-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

NADH-cytochrome b5 reductase 1, NADH cytochrome b5 reductase 1, Humb5R2, EC:1.6.2.2, cytochrome b5 reductase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, human brain tissue, PC-3 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11807-1-AP targets CYB5R1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CYB5R1 fusion protein Ag2475 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome b5 reductase 1
别名 NADH-cytochrome b5 reductase 1, NADH cytochrome b5 reductase 1, Humb5R2, EC:1.6.2.2, cytochrome b5 reductase 1
计算分子量 305 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC018732
基因名称 CYB5R1
Gene ID (NCBI) 51706
RRIDAB_2089968
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHQ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYB5R1 antibody 11807-1-APDownload protocol
IHC protocol for CYB5R1 antibody 11807-1-APDownload protocol
IF protocol for CYB5R1 antibody 11807-1-APDownload protocol
IP protocol for CYB5R1 antibody 11807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Free Radic Res

Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells

Authors - Daisaku Yoshihara
View Article
humanWB

J Transl Med

Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy

Authors - Tingting Zhao
View Article