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CYB5R1 Polyclonal antibody

CYB5R1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11807-1-AP

Print datasheet

Synonyms

B5R.1, CYB5R1, cytochrome b5 reductase 1, humb5R2, NADH cytochrome b5 reductase 1, NQO3A2

验证数据展示

  • WB analysis of human placenta using 11807-1-AP

    WB analysis of human placenta using 11807-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11807-1-AP (CYB5R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11807-1-AP

    IHC staining of human breast cancer using 11807-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11807-1-AP (CYB5R1 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of human placenta using 11807-1-AP

    IP experiment of human placenta using 11807-1-AP

    IP result of anti-CYB5R1 (IP:11807-1-AP, 4ug; Detection:11807-1-AP 1:1000) with human placenta tissue lysate 1520ug.

  • IF Staining of PC-3 using 11807-1-AP

    IF Staining of PC-3 using 11807-1-AP

    Immunofluorescent analysis of PC-3 cells using 11807-1-AP (CYB5R1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, human brain tissue, PC-3 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11807-1-AP targets CYB5R1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CYB5R1 fusion protein Ag2475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome b5 reductase 1
Synonyms B5R.1, CYB5R1, cytochrome b5 reductase 1, humb5R2, NADH cytochrome b5 reductase 1, NQO3A2
Calculated Molecular Weight 305 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC018732
Gene Symbol CYB5R1
Gene ID (NCBI) 51706
RRIDAB_2089968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYB5R1 antibody 11807-1-APDownload protocol
IHC protocol for CYB5R1 antibody 11807-1-APDownload protocol
IF protocol for CYB5R1 antibody 11807-1-APDownload protocol
IP protocol for CYB5R1 antibody 11807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Free Radic Res

Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells

Authors - Daisaku Yoshihara
View Article
humanWB

J Transl Med

Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy

Authors - Tingting Zhao
View Article