验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, human brain tissue, PC-3 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
11807-1-AP targets CYB5R1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2475 Product name: Recombinant human CYB5R1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC018732 Sequence: MGIQTSPVLLASLGVGLVTLLGLAVGSYLVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome b5 reductase 1 |
| 别名 | b5R.1, cytochrome b5 reductase 1, EC:1.6.2.2, Humb5R2, NADH cytochrome b5 reductase 1 |
| 计算分子量 | 305 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC018732 |
| 基因名称 | CYB5R1 |
| Gene ID (NCBI) | 51706 |
| RRID | AB_2089968 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| IHC protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| IP protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| WB protocol for CYB5R1 antibody 11807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Res Iron deficiency aggravates DMNQ-induced cytotoxicity via redox cycling in kidney-derived cells | ||
J Transl Med Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy | ||
Free Radic Biol Med Downregulation of the SREBP pathways and disruption of redox status by 25-hydroxycholesterol predispose cells to ferroptosis
|
