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验证数据展示

  • WB analysis of K-562 using 27963-1-AP

    WB analysis of K-562 using 27963-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27963-1-AP (CXXC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27963-1-AP

    IHC staining of human breast cancer using 27963-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27963-1-AP (CXXC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXXC1 Polyclonal antibody

CXXC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27963-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

2410002I16Rik, 5830420C16Rik, CFP1, CGBP, CpG binding protein, CXXC finger 1 (PHD domain), CXXC1, hCGBP, HsT2645, PCCX1, PHF18, SPP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27963-1-AP targets CXXC1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag27558

Product name: Recombinant human CXXC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC015733

Sequence: MEGDGSDPEPPDAGEDSKSENGENAPIYCICRKPDINCFMIGCDNCNEWFHGDCIRITEKMAKAIREWYCRECREKDPKLEIRYRHKKSRERDGNERDSSEPRDEGGGRKRPVPDPDLQRRAGSGTGVGAMLARGSASPHKSSPQPLVATPSQHHQQQQQQIKRSARMCGECEACRRTEDCGHCDFCRDMKKFGGPNKIRQKCRLRQCQLRARESY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CXXC finger 1 (PHD domain)
别名 2410002I16Rik, 5830420C16Rik, CFP1, CGBP, CpG binding protein, CXXC finger 1 (PHD domain), CXXC1, hCGBP, HsT2645, PCCX1, PHF18, SPP1
计算分子量 76 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC015733
基因名称 CXXC1
Gene ID (NCBI) 30827
RRIDAB_2881023
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0U4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CXXC1 antibody 27963-1-APDownload protocol
IHC protocol for CXXC1 antibody 27963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols