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验证数据展示

  • WB analysis using 28239-1-AP (same clone as 28239-1-PBS)

    WB analysis using 28239-1-AP (same clone as 28239-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28239-1-AP (same clone as 28239-1-PBS)

    IHC staining of mouse brain using 28239-1-AP (same clone as 28239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28239-1-AP (CXCR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 28239-1-AP (same clone as 28239-1-PBS)

    WB analysis of Jurkat using 28239-1-AP (same clone as 28239-1-PBS)

    WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells. This data was developed using the same antibody clone with 28239-1-PBS in a different storage buffer formulation.

CXCR5 Polyclonal antibody, PBS Only

CXCR5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28239-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28239-1-PBS targets CXCR5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28271

Product name: Recombinant human CXCR5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 241-372 aa of NM_001716

Sequence: MVVHRLRQAQRRPQRQKAVRVAILVTSIFFLCWSPYHIVIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLLTKLGCTGPASLCQLFPSWRRSSLSESENATSLTTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 5
别名 MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1
计算分子量 42 kDa
观测分子量33 kDa
GenBank蛋白编号NM_001716
基因名称 CXCR5
Gene ID (NCBI) 643
RRIDAB_3086040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32302
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

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