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  • Featured Product
  • KD/KO Validated

CXCR3B-specific Monoclonal antibody, PBS Only

CXCR3B-specific Monoclonal Antibody for WB,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

1B2D6

Cat No : 60065-1-PBS

Print datasheet

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

验证数据展示

  • FC experiment of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

    FC experiment of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human CXCR3B-specific (60065-1-Ig, Clone:1B2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

    WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

产品信息

60065-1-PBS targets CXCR3B-specific in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,FC
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 3
Synonyms CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR
Calculated Molecular Weight 46 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberAF469635
Gene Symbol CXCR3
Gene ID (NCBI) 2833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49682
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.