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验证数据展示

  • WB analysis using 85144-5-RR (same clone as 85144-5-PBS)

    WB analysis using 85144-5-RR (same clone as 85144-5-PBS)

    Rat liver tissue was subjected to SDS PAGE followed by western blot with 85144-5-RR (CXCR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85144-5-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 85144-5-RR (same clone as 85144-5-PBS)

    IHC staining of rat spleen using 85144-5-RR (same clone as 85144-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85144-5-RR (CXCR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85144-5-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 85144-5-RR (same clone as 85144-5-PBS)

    IHC staining of rat spleen using 85144-5-RR (same clone as 85144-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85144-5-RR (CXCR2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85144-5-PBS in a different storage buffer formulation.

Mouse CXCR2 Recombinant monoclonal antibody, PBS Only

CXCR2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85144-5-PBS

产品说明书

CloneNo. 242766H6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Indirect ELISA

别名

242766H6, CD182, Cmkar2, C-X-C chemokine receptor type 2, CXC-R2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85144-5-PBS targets CXCR2 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2228

Product name: Recombinant Mouse CXCR2 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 1-47 aa of NM_009909.3

Sequence: MGEFKVDKFNIEDFFSGDLDIFNYSSGMPSILPDAVPCHSENLEINS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 8 receptor, beta
别名 242766H6, CD182, Cmkar2, C-X-C chemokine receptor type 2, CXC-R2
计算分子量40 kDa
观测分子量45 kDa
GenBank蛋白编号NM_009909.3
基因名称 Cxcr2
Gene ID (NCBI) 12765
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35343
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CXCR2, also named as Cmkar2, Gpcr16, Il8rb or CD182, belongs to the G-protein coupled receptor 1 family. CXCR2 is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. CXCR2 binds to IL-8 with high affinity. CXCR2 also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.

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