×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 33296-1-AP

    WB analysis using 33296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33296-1-AP (CXCL7/PPBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse placenta using 33296-1-AP

    IHC staining of mouse placenta using 33296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 33296-1-AP (PPBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 33296-1-AP

    IHC staining of mouse placenta using 33296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 33296-1-AP (PPBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCL7/PPBP Polyclonal antibody

CXCL7/PPBP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Chemokine subfamily B Cys-X-Cys Platelet basic protein Thymus chemokine 1, Cxcl7, Ppbp

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse serum, mouse spleen tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33296-1-AP targets CXCL7/PPBP in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2806

Product name: Recombinant Mouse CXCL7/PPBP protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 48-109 aa of NM_023785.3

Sequence: IELRCRCTNTISGIPFNSISLVNVYRPGVHCADVEVIATLKNGQKTCLDPNAPGVKRIVMKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pro-platelet basic protein
别名 Chemokine subfamily B Cys-X-Cys Platelet basic protein Thymus chemokine 1, Cxcl7, Ppbp
计算分子量12 kDa
观测分子量 10-12 kDa
GenBank蛋白编号NM_023785.3
基因名称 Ppbp
Gene ID (NCBI) 57349
RRIDAB_3742932
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9EQI5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

实验方案

Product Specific Protocols
IHC protocol for CXCL7/PPBP antibody 33296-1-APDownload protocol
WB protocol for CXCL7/PPBP antibody 33296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...