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验证数据展示

  • WB analysis using 10809-1-AP

    WB analysis using 10809-1-AP

    Untreated, PMA treated A549 cells were subjected to SDS PAGE followed by western blot with 10809-1-AP (CXCL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10809-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 10809-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10809-1-AP (CXCL5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXCL5 antibody included

LIX/CXCL5 Polyclonal antibody

LIX/CXCL5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10809-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CXCL5, ENA-78(1-78), CXCL-5, CXCL 5, C-X-C motif chemokine 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPMA treated A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CXCL5 Ready-To-Use IHC Kit. CXCL5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10809-1-AP targets LIX/CXCL5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
免疫原 LIX/CXCL5 fusion protein Ag1079 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 5
别名 CXCL5, ENA-78(1-78), CXCL-5, CXCL 5, C-X-C motif chemokine 5
计算分子量 12 kDa
观测分子量8 kDa
GenBank蛋白编号BC008376
基因名称 CXCL5
Gene ID (NCBI) 6374
RRIDAB_2292366
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.

实验方案

Product Specific Protocols
WB protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
IHC protocol for LIX/CXCL5 antibody 10809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Stem Cells

Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs: Insights from Single-Cell RNA Sequencing

Authors - Peng Huang
View Article