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Species Reactivity
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验证数据展示

  • WB analysis using 29589-1-AP

    WB analysis using 29589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29589-1-AP (CXCL16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CXCL16 Polyclonal antibody

CXCL16 Polyclonal Antibody for WB, ELISA
Cat No. 29589-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29589-1-AP targets CXCL16 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 CXCL16 fusion protein Ag30981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 16
别名 C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16
计算分子量 273 aa, 30 kDa
观测分子量28 kDa , 50-55 kDa
GenBank蛋白编号BC017588
基因名称 CXCL16
Gene ID (NCBI) 58191
RRIDAB_3086142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2A7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCL16 was discovered as a ligand for CXC chemokine receptor (CXCR) 6. CXCL16 is a membrane-bound chemokine that consists of four distinct domains: the chemokine domain, mucin-like domain, transmembrane domain and ad cytoplasmic. After cleavage, soluble CXCL16 acts as a chemoattractant for activated CD8 T cells, NKT cells and Th1-polarized T cells that express CXCR6. CXCL16 expression has been studied mainly in macrophages and dendritic cells. CXCL16 production by non-immune cells remains controversial. CXCL16 was expressed as two different forms: a 50 kDa form and a 28 kDa form, and the 50 kDa may be the glycosylated form (PMID: 17855433, 33552057). (PMID: 17855433, 33552057).

实验方案

Product Specific Protocols
WB protocol for CXCL16 antibody 29589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols