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验证数据展示

  • WB analysis of TT using 87928-3-RR

    WB analysis of TT using 87928-3-RR

    TT cells were subjected to SDS PAGE followed by western blot with 87928-3-RR (CXCL13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87928-3-RR

    IHC staining of human tonsillitis using 87928-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87928-3-RR (CXCL13 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 87928-3-RR

    IHC staining of human tonsillitis using 87928-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87928-3-RR (CXCL13 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCL13/BCA1 Recombinant monoclonal antibody

CXCL13/BCA1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87928-3-RR

产品说明书

CloneNo. 253456A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CXCL13, B cell-attracting chemokine 1, BCA 1, BCA1, BCA-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTT cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87928-3-RR targets CXCL13/BCA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg7258

Product name: Recombinant human CXCL13 protein

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 23-109 aa of NM_006419.2

Sequence: VLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 13
别名 CXCL13, B cell-attracting chemokine 1, BCA 1, BCA1, BCA-1
计算分子量13 kDa
观测分子量13 kDa
GenBank蛋白编号NM_006419.2
基因名称 CXCL13/BCA1
Gene ID (NCBI) 10563
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ53X90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCL13 (C-X-C motif chemokine ligand 13), also known as B cell-attracting chemokine 1 (BCA-1) or B-lymphocyte chemoattractant (BLC), is a small cytokine belonging to the CXC chemokine family. It was independently discovered by two research groups in 1998-Legler et al. identified it as BCA-1, while Gunn et al. described it as BLC. CXCL13 is primarily secreted by follicular dendritic cells (FDCs) within B-cell follicles of secondary lymphoid organs, including lymph nodes, spleen, and Peyer's patches.

实验方案

Product Specific Protocols
IHC protocol for CXCL13/BCA1 antibody 87928-3-RRDownload protocol
WB protocol for CXCL13/BCA1 antibody 87928-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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