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验证数据展示

  • WB analysis using 87669-1-RR (same clone as 87669-1-PBS)

    WB analysis using 87669-1-RR (same clone as 87669-1-PBS)

    HEK-293T cells and mouse Cxcl12 transfected HEK-293T cells lysates were subjected to SDS PAGE followed by western blot with 87669-1-RR (CXCL12/SDF-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87669-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87669-1-RR (same clone as 87669-1-PBS)

    IHC staining of mouse spleen using 87669-1-RR (same clone as 87669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87669-1-RR (CXCL12/SDF-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87669-1-PBS in a different storage buffer formulation.

  • Affinity of 87669-1-RR

    Affinity of 87669-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87669-1-RR against Mouse CXCL12/SDF-1 were performed. The affinity constant is 4.03 nM.

Mouse CXCL12/SDF-1 Recombinant monoclonal antibody, PBS Only

CXCL12/SDF-1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87669-1-PBS

产品说明书

CloneNo. 252850A8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

Cxcl12, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, C-X-C motif chemokine 12, CXCL 12, hIRH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87669-1-PBS targets CXCL12/SDF-1 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2402

Product name: recombinant mouse Cxcl12 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-89 aa of BC020297

Sequence: KPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 12
别名 Cxcl12, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, C-X-C motif chemokine 12, CXCL 12, hIRH
计算分子量 11 kDa
观测分子量15 kDa
GenBank蛋白编号BC020297
基因名称 Cxcl12
Gene ID (NCBI) 20315
RRIDAB_3745686
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP40224
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ).

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