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验证数据展示

  • WB analysis using 86186-1-RR

    WB analysis using 86186-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86186-1-RR

    WB analysis using 86186-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 86186-1-RR

    IHC staining of mouse heart using 86186-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat heart using 86186-1-RR

    IHC staining of rat heart using 86186-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86186-1-RR

    Affinity and Kinetic Characterization of 86186-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86186-1-RR against Human CXADR were performed. The affinity constant is 96.1 pM.

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CXADR Recombinant monoclonal antibody

CXADR Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86186-1-RR

产品说明书

CloneNo. 250811C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHaCaT cells, mouse heart tissue, rat heart tissue, mouse small intestine tissue, BxPC-3 cells
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86186-1-RR targets CXADR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34346

Product name: Recombinant human CXADR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-365 aa of BC003684

Sequence: MALLLCFVLLCGVVDFARSLSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQVIILYSGDKIYDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKIHLVVLVKPSGARCYVDGSEEIGSDFKIKCEPKEGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISVKNASSEYSGTYSCTVRNRVGSDQCLLRLNVVPPSNKAGLIAGAIIGTLLALALIGLIIFCCRKKRREEKYEKEVHHDIREDVPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQVPSEDFERTPQSPTLPPAKVAAPNLSRMGAIPVMIPAQSKDGSIV*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coxsackie virus and adenovirus receptor
别名 CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR
计算分子量 365 aa, 40 kDa
观测分子量45-55 kDa
GenBank蛋白编号BC003684
基因名称 CXADR
Gene ID (NCBI) 1525
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78310
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).

实验方案

Product Specific Protocols
WB protocol for CXADR antibody 86186-1-RRDownload protocol
IHC protocol for CXADR antibody 86186-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols