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CoraLite® Plus 488-conjugated CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F5D5

Cat No : CL488-60339

Print datasheet

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

验证数据展示

  • FC experiment of PC-3 using CL488-60339

    FC experiment of PC-3 using CL488-60339

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CX3CL1 (CL488-60339, Clone:2F5D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 60339-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60339 targets CX3CL1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen CX3CL1 fusion protein Ag0161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chemokine (C-X3-C motif) ligand 1
Synonyms ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001163
Gene Symbol CX3CL1
Gene ID (NCBI) 6376
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78423
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CX3CL1 antibody CL488-60339Download protocol
Standard Protocols
Click here to view our Standard Protocols