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验证数据展示

  • WB analysis using 85353-2-RR (same clone as 85353-2-PBS)

    WB analysis using 85353-2-RR (same clone as 85353-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85353-2-RR (CX3CL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85353-2-RR (same clone as 85353-2-PBS)

    IHC staining of mouse brain using 85353-2-RR (same clone as 85353-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85353-2-RR (CX3CL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CX3CL1 antibody included
  • IHC staining of rat brain using 85353-2-RR (same clone as 85353-2-PBS)

    IHC staining of rat brain using 85353-2-RR (same clone as 85353-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85353-2-RR (CX3CL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CX3CL1 antibody included

CX3CL1 Recombinant monoclonal antibody, PBS Only

CX3CL1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85353-2-PBS

产品说明书

CloneNo. 251665G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

A-152E5.2, ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3C membrane-anchored chemokine

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CX3CL1 Ready-To-Use IHC Kit. CX3CL1 primary antibody included.

产品信息

85353-2-PBS targets CX3CL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6637

Product name: recombinant human CX3CL1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-341 aa of BC016164

Sequence: QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X3-C motif) ligand 1
别名 A-152E5.2, ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3C membrane-anchored chemokine
计算分子量42 kDa
观测分子量55-75 kDa
GenBank蛋白编号BC016164
基因名称 CX3CL1
Gene ID (NCBI) 6376
RRIDAB_3744158
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78423
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).

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