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CWF19L2 Polyclonal antibody

CWF19L2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24872-1-AP

Print datasheet

Synonyms

CWF19 like protein 2, CWF19L2

验证数据展示

  • WB analysis of HeLa using 24872-1-AP

    WB analysis of HeLa using 24872-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24872-1-AP

    IHC staining of human kidney using 24872-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24872-1-AP (CWF19L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24872-1-AP is CWF19L2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen CWF19L2 fusion protein Ag21738 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CWF19-like 2, cell cycle control (S. pombe)
Synonyms CWF19 like protein 2, CWF19L2
Calculated Molecular Weight 894 aa, 104 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC110442
Gene Symbol CWF19L2
Gene ID (NCBI) 143884
RRIDAB_2879769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TBE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CWF19L2 antibody 24872-1-APDownload protocol
IHC protocol for CWF19L2 antibody 24872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols