验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
26898-1-AP targets CWC22 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25466 Product name: Recombinant human CWC22 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 559-905 aa of BC093954 Sequence: SLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSRGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CWC22 spliceosome-associated protein homolog (S. cerevisiae) |
| 别名 | Pre-mRNA-splicing factor CWC22 homolog, NCM, fSAPb, EIF4GL |
| 计算分子量 | 908 aa, 106 kDa |
| 观测分子量 | 105-110 kDa |
| GenBank蛋白编号 | BC093954 |
| 基因名称 | CWC22 |
| Gene ID (NCBI) | 57703 |
| RRID | AB_2880674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HCG8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CWC22 antibody 26898-1-AP | Download protocol |
| WB protocol for CWC22 antibody 26898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
