CWC22 Polyclonal antibody
CWC22 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
The immunogen of 26898-1-AP is CWC22 Fusion Protein expressed in E. coli.
| Tested Applications | WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CWC22 fusion protein Ag25466 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CWC22 spliceosome-associated protein homolog (S. cerevisiae) |
| Synonyms | CWC22, EIF4GL, fSAPb, KIAA1604, NCM, Nucampholin homolog |
| Calculated Molecular Weight | 908 aa, 106 kDa |
| Observed Molecular Weight | 105-110 kDa |
| GenBank Accession Number | BC093954 |
| Gene Symbol | CWC22 |
| Gene ID (NCBI) | 57703 |
| RRID | AB_2880674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CWC22, Pre-mRNA-splicing factor CWC22 homolog, is a 908 amino acid protein, which contains 1 MI domain and 1 MIF4G domain and belongs to the CWC22 family. CWC22 is required for pre-mRNA splicing and for exon-junction complex (EJC) assembly and hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CWC22 antibody 26898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. |
