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验证数据展示

  • WB analysis using 15610-1-AP (same clone as 15610-1-PBS)

    WB analysis using 15610-1-AP (same clone as 15610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15610-1-AP (CUTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15610-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 15610-1-AP (same clone as 15610-1-PBS)

    IF Staining of A549 using 15610-1-AP (same clone as 15610-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CUTA antibody (15610-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15610-1-PBS in a different storage buffer formulation.

CUTA Polyclonal antibody, PBS Only

CUTA Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 15610-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Acetylcholinesterase-associated protein, ACHAP, C6orf82, Protein CutA

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15610-1-PBS targets CUTA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8000

Product name: Recombinant human CUTA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-156 aa of BC005890

Sequence: MPALLPVASRLLLLPRVLLTMASGSPPTQPSPASDSGSGYVPGSVSAAFVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIALPVEQGNFPYLQWVRQVTESVSDSITVLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cutA divalent cation tolerance homolog (E. coli)
别名 Acetylcholinesterase-associated protein, ACHAP, C6orf82, Protein CutA
计算分子量 19 kDa
观测分子量 15 kDa
GenBank蛋白编号BC005890
基因名称 CUTA
Gene ID (NCBI) 51596
RRIDAB_3669214
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60888
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CUTA also known as ACHAP, functions in cellular copper sensitivity and in the processing and trafficking of membrane proteins. CUTA, the mammalian CutA divalent cation tolerance homolog (E. coli), has been proposed to mediate acetylcholinesterase activity and copper homeostasis. Human CUTA has several variants that differ in N-terminal length and are separated as heavy (H) and light (L) components. The H component of human CUTA was associated with the membrane fraction, whereas the L component of human CUTA was in the cytosol(PMID: 22351782).

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