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验证数据展示

  • WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

    various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

    WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4B antibody included
  • Featured Product
  • KD/KO验证

CUL4B Monoclonal antibody, PBS Only

CUL4B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60151-1-PBS

产品说明书

CloneNo. 4E10B10

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

4E10B10, cullin 4B, Cullin-4B, KIAA0695

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

产品信息

60151-1-PBS targets CUL4B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag3563

Product name: Recombinant human CUL4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC036216

Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGIL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 4B
别名 4E10B10, cullin 4B, Cullin-4B, KIAA0695
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_10641034
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13620
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).