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验证数据展示

  • WB analysis using 20882-1-AP

    WB analysis using 20882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 20882-1-AP

    WB analysis of HeLa cells using 20882-1-AP

    WB result of CUL4B antibody (20882-1-AP, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

  • IP experiment of mouse brain using 20882-1-AP

    IP experiment of mouse brain using 20882-1-AP

    IP result of anti-CUL4B (IP:20882-1-AP, 4ug; Detection:20882-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human tonsillitis using 20882-1-AP

    IHC staining of human tonsillitis using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL4B antibody included
  • IHC staining of human tonsillitis using 20882-1-AP

    IHC staining of human tonsillitis using 20882-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUL4B antibody included
  • IF Staining of HeLa using 20882-1-AP

    IF Staining of HeLa using 20882-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 20882-1-AP

    IF Staining of HepG2 using 20882-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20882-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

KIAA0695, Cullin-4B, cullin 4B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, Jurkat cells, Y79 cells, SH-SY5Y cells, HeLa cells, rat thymus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20882-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用IF, IP
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 CUL4B fusion protein Ag15024 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 4B
别名 KIAA0695, Cullin-4B, cullin 4B
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_10694831
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13620
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

实验方案

Product Specific Protocols
WB protocol for CUL4B antibody 20882-1-APDownload protocol
IHC protocol for CUL4B antibody 20882-1-APDownload protocol
IF protocol for CUL4B antibody 20882-1-APDownload protocol
IP protocol for CUL4B antibody 20882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Neuropsychopharmacology

Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.

Authors - Gonca Bayraktar
  • KD Validated
View Article
mouseIP

Front Immunol

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Authors - Nianyin Lv
View Article
mouseIF

Cells

Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis.

Authors - Yan Yin
View Article