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验证数据展示

  • WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    WB analysis using 66038-1-Ig (same clone as 66038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66038-1-Ig (CUL4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP experiment of MCF-7 using 66038-1-Ig (same clone as 66038-1-PBS)

    IP result of anti-CUL4A (IP:66038-1-Ig, 4ug; Detection:66038-1-Ig 1:500) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4A antibody included
  • IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    IHC staining of human heart using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66038-1-Ig (CUL4A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUL4A antibody included
  • IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    IF Staining of HepG2 using 66038-1-Ig (same clone as 66038-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66038-1-Ig (CUL4A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66038-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CUL4A Monoclonal antibody, PBS Only

CUL4A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66038-1-PBS

产品说明书

CloneNo. 1A7F12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, monkey

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

1A7F12, CUL 4A, CUL-4A, cullin 4A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CUL4A Ready-To-Use IHC Kit. CUL4A primary antibody included.

产品信息

66038-1-PBS targets CUL4A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, monkey
免疫原

CatNo: Ag18089

Product name: Recombinant human CUL4A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 460-759 aa of BC008308

Sequence: RLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 4A
别名 1A7F12, CUL 4A, CUL-4A, cullin 4A
计算分子量 77 kDa
观测分子量 77 kDa, 88 kDa
GenBank蛋白编号BC008308
基因名称 CUL4A
Gene ID (NCBI) 8451
RRIDAB_11042446
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13619
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

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