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验证数据展示

  • WB analysis using 12921-1-AP

    WB analysis using 12921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12921-1-AP

    IHC staining of human breast cancer using 12921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CUGBP2 antibody included
  • IF Staining of HepG2 using 12921-1-AP

    IF Staining of HepG2 using 12921-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12921-1-AP (CUGBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse skeletal muscle using 12921-1-AP

    IP experiment of mouse skeletal muscle using 12921-1-AP

    IP result of anti-CUGBP2 (IP:12921-1-AP, 3ug; Detection:12921-1-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

CUGBP2 Polyclonal antibody

CUGBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12921-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue, K-562 cells, MCF-7 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CUGBP2 Ready-To-Use IHC Kit. CUGBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12921-1-AP targets CUGBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag3594

Product name: Recombinant human CUGBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 175-521 aa of BC036391

Sequence: STRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGTINTPRSKRLLLPKDNNGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CUG triplet repeat, RNA binding protein 2
别名 CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3
计算分子量 56 kDa
观测分子量 50 kDa
GenBank蛋白编号BC036391
基因名称 CUGBP2
Gene ID (NCBI) 10659
RRIDAB_2230041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95319
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.

实验方案

Product Specific Protocols
IF protocol for CUGBP2 antibody 12921-1-APDownload protocol
IHC protocol for CUGBP2 antibody 12921-1-APDownload protocol
IP protocol for CUGBP2 antibody 12921-1-APDownload protocol
WB protocol for CUGBP2 antibody 12921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Aberrant Splicing Signatures Underpin Oligodendrocyte Damage in ALS and Neuron Loss in FTD.

Authors - Chen Du
  • KD Validated
View Article
humanWB,IF

Pharmacol Res

Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway

Authors - Ying Zhang
View Article
humanIHC

Oncogenesis

Investigation of lncRNA expression in newly diagnosed multiple myeloma reveals a LINC01432-CELF2 axis as an inhibitor of apoptosis.

Authors - Richa Mishra
View Article
humanWB

Oncotarget

Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.

Authors - Bo Li
View Article
humanWB

Exp Biol Med (Maywood)

Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.

Authors - Liuqing Ge
View Article
mouseIHC

Brain Res

Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.

Authors - Otsuka Noboru N
View Article
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