验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17893-1-AP targets CTTNBP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12219 Product name: Recombinant human CTTNBP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1312-1663 aa of BC106000 Sequence: LSVWRQLNSCLARLGTPEALLGPKYFLSCPVVPGHAQVTVKWMSKLWNGVIAPRVQEAILSRASVKRQPGFGQTTAKRHPSQGQQAVVKAALSILLNKAVLHGCPLPRAELDQHTADFKGGSFPLSIVSSYNTCNKKKGESGAWRKVNTSPRRKSGRFSLPTWNKPDLSTEGMKNKTISQLNCNRNASLSKQKSLENDLSLTLNLDQRLSLGSDDEADLVKELQSMCSSKSESDISKIADSRDDLRMFDSSGNNPVLSATINNLRMPVSQKEVSPLSSHQTTECSNSKSKTELGVSRVKSFLPVPRSKVTQCSQNTKRSSSSSNTRQIEINNNSKEVNWNLHKNEHLEKPNK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cortactin binding protein 2 |
| 别名 | C7orf8, Cortactin-binding protein 2, CortBP2, KIAA1758, Orf4 |
| 计算分子量 | 1663 aa, 181 kDa |
| 观测分子量 | 181 kDa |
| GenBank蛋白编号 | BC106000 |
| 基因名称 | CTTNBP2 |
| Gene ID (NCBI) | 83992 |
| RRID | AB_2230211 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WZ74 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cortactin Binding Protein 2 (CTTNBP2) is a cytoskeleton regulator predominantly expressed in neurons and highly enriched at dendritic spines. CTTNBP2 controls the synaptic expression of a set of zinc-regulated autism-associated genes and influences NMDAR function and signaling (PMID: 32492416).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CTTNBP2 antibody 17893-1-AP | Download protocol |
| IP protocol for CTTNBP2 antibody 17893-1-AP | Download protocol |
| WB protocol for CTTNBP2 antibody 17893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition. | ||
Dev Cell Single-cell MultiOmics and spatial transcriptomics demonstrate neuroblastoma developmental plasticity | ||
Mod Pathol Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets. | ||
Sci Adv Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression |
