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验证数据展示

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 14296-1-AP

    IP experiment of HEK-293 using 14296-1-AP

    IP result of anti-CTSO (IP:14296-1-AP, 4ug; Detection:14296-1-AP 1:300) with HEK-293 cells lysate 3600ug.

CTSO Polyclonal antibody

CTSO Polyclonal Antibody for IP, IHC, ELISA
Cat No. 14296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IP, IHC, ELISA

别名

cathepsin O, CTSO, CTSO1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14296-1-AP targets CTSO in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5651

Product name: Recombinant human CTSO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-321 aa of BC049206

Sequence: MDVRALPWLPWLLWLLCRGGGDADSRAPFTPTWPRSREREAAAFRESLNRHRYLNSLFPSENSTAFYGINQFSYLFPEEFKAIYLRSKPSKFPRYSAEVHMSIPNVSLPLRFDWRDKQVVTQVRNQQMCGGCWAFSVVGAVESAYAIKGKPLEDLSVQQVIDCSYNNYGCNGGSTLNALNWLNKMQVKLVKDSEYPFKAQNGLCHYFSGSHSGFSIKGYSAYDFSDQEDEMAKALLTFGPLVVIVDAVSWQDYLGGIIQHHCSSGEANHAVLITGFDKTGSTPYWIVRNSWGSSWGVDGYAHVKMGSNVCGIADSVSSIFV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin O
别名 cathepsin O, CTSO, CTSO1
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC049206
基因名称 CTSO
Gene ID (NCBI) 1519
RRIDAB_2878042
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43234
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CTSO antibody 14296-1-APDownload protocol
IP protocol for CTSO antibody 14296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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