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验证数据展示

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14296-1-AP

    IHC staining of human ovary tumor using 14296-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14296-1-AP (CTSO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 14296-1-AP

    IP experiment of HEK-293 using 14296-1-AP

    IP result of anti-CTSO (IP:14296-1-AP, 4ug; Detection:14296-1-AP 1:300) with HEK-293 cells lysate 3600ug.

CTSO Polyclonal antibody

CTSO Polyclonal Antibody for IP, IHC, ELISA
Cat No. 14296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IP, IHC, ELISA

别名

cathepsin O, CTSO, CTSO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14296-1-AP targets CTSO in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CTSO fusion protein Ag5651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin O
别名 cathepsin O, CTSO, CTSO1
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC049206
基因名称 CTSO
Gene ID (NCBI) 1519
RRIDAB_2878042
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43234
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CTSO antibody 14296-1-APDownload protocol
IP protocol for CTSO antibody 14296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols