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验证数据展示

  • WB analysis of HCT 116 using 21327-1-AP

    WB analysis of HCT 116 using 21327-1-AP

    WB result of Cathepsin D antibody (21327-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and sg-Cathepsin D transfected HCT-116 cells.

  • WB analysis using 21327-1-AP

    WB analysis using 21327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 21327-1-AP

    IHC staining of human hepatocirrhosis using 21327-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human hepatocirrhosis using 21327-1-AP

    IHC staining of human hepatocirrhosis using 21327-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • WB analysis using 21327-1-AP

    WB analysis using 21327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21327-1-AP

    IF Staining of MCF-7 using 21327-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of A431 using 21327-1-AP

    IF Staining of A431 using 21327-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21327-1-AP

产品说明书

引用文献(219)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (4)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HCT 116 cells, HuH-7 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21327-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, chicken, bovine
免疫原

CatNo: Ag15254

Product name: Recombinant human Cathepsin D protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 59-412 aa of BC016320

Sequence: VPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin D
别名 CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD
计算分子量 412 aa, 45 kDa
观测分子量 32 kDa, 48 kDa, 52 kDa
GenBank蛋白编号BC016320
基因名称 Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_10733646
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07339
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 28~32-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

实验方案

Product Specific Protocols
IF protocol for Cathepsin D antibody 21327-1-APDownload protocol
IHC protocol for Cathepsin D antibody 21327-1-APDownload protocol
WB protocol for Cathepsin D antibody 21327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,human

Cell Metab

ABCG2 is an itaconate exporter that limits antibacterial innate immunity by alleviating TFEB-dependent lysosomal biogenesis

Authors - Chao Chen
View Article
humanWB

Cancer Commun (Lond)

HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression

Authors - Lin He
View Article
mouseWB

Nat Metab

mTORC1 regulates a lysosome-dependent adaptive shift in intracellular lipid species

Authors - Aaron M Hosios
View Article
mouseIF

Bioact Mater

Biomimetic stress granules replenish lysosomal repair to reinstate macrophage immunometabolic antibacterial programs.

Authors - Shenzhi Zhao
View Article
IHC

Nat Cell Biol

Mitochondrial-derived vesicles drive budding-type fission of damaged lysosomes

Authors - Yuanchun Luo
View Article

Nat Neurosci

Mitochondrial accumulation and lysosomal dysfunction result in mitochondrial plaques in Alzheimer's disease

Authors - Xiuli Dan
View Article
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