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验证数据展示

  • WB analysis using 30289-1-AP

    WB analysis using 30289-1-AP

    human adipose tissue was subjected to SDS PAGE followed by western blot with 30289-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 30289-1-AP

    IHC staining of mouse heart using 30289-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30289-1-AP

    IHC staining of mouse kidney using 30289-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTRP1 Polyclonal antibody

CTRP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30289-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP, UNQ310/PRO353

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman adipose tissue
Positive IHC detected inmouse heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30289-1-AP targets CTRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31779

Product name: Recombinant human C1QTNF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-281 aa of NM_030968

Sequence: RVPHVQGEQQEWEGTEELPSPPDHAERAEEQHEKYRPSQDQGLPASRCLRCCDPGTSMYPATAVPQINITILKGEKGDRGDRGLQGKYGKTGSAGARGHTGPKGQKGSMGAPGERCKSHYAAFSVGRKKPMHSNHYYQTVIFDTEFVNLYDHFNMFTGKFYCYVPGLYFFSLNVHTWNQKETYLHIMKNEEEVVILFAQVGDRSIMQSQSLMLELREQDQVWVRLYKGERENAIFSEELDTYITFSGYLVKHATEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C1q and tumor necrosis factor related protein 1
别名 C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP, UNQ310/PRO353
计算分子量32KD
观测分子量 35 kDa
GenBank蛋白编号NM_030968
基因名称 CTRP1
Gene ID (NCBI) 114897
RRIDAB_3669708
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

实验方案

Product Specific Protocols
IHC protocol for CTRP1 antibody 30289-1-APDownload protocol
WB protocol for CTRP1 antibody 30289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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