CTNS Polyclonal antibody
CTNS Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IF detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
13085-1-AP targets CTNS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CTNS fusion protein Ag3904 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cystinosis, nephropathic |
| Synonyms | CTNS, CTNS LSB, Cystinosin, cystinosis, nephropathic, PQLC4 |
| Calculated Molecular Weight | 400aa,45 kDa; 367aa,42 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC032850 |
| Gene Symbol | CTNS |
| Gene ID (NCBI) | 1497 |
| RRID | AB_2230084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60931 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CTNS or cystinosin is a lysosomal membrane protein which comprises with seven transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a cytosolic C-terminal GYDQL sorting motif. It functions as a H+-driven cystine transporter responsible for cystine export from lysosomes. The mutation of CTNS gene cause an inherited disorder, cystinosis, characterized by defective lysosomal efflux of cystine. This antibody was raised against the N-terminal region of human cystinosin. Two isoforms of CTNS exist due to alternative splicing events and both of them can be detected through this antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTNS antibody 13085-1-AP | Download protocol |
| IF protocol for CTNS antibody 13085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Reduced phosphate transport in the renal proximal tubule cells in cystinosis is due to decreased expression of transporters rather than an energy defect. | ||
Biochem Biophys Res Commun Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. | ||
J Immunol Methods Characterization of a rat monoclonal antibody raised against ferroptotic cells. |
