验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HepG2 cells, HeLa cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32926-1-AP targets CTNNAL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38629 Product name: Recombinant human CTNNAL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 290-535 aa of NM_003798 Sequence: ISIFTGIKEFKMNIEALRENLYFQSKENLSVTLEVILERMEDFTDSAYTSHEHRERILELSTQARMELQQLISVWIQAQSKKTKSIAEELELSILKISHSLNELKKELHSTATQLAADLLKYHADHVVLKALKLTGVEGNLEALAEYACKLSEQKEQLVETCRLLRHISGTEPLEITCIHAEETFQVTGQQIISAAETLTLHPSSKIAKENLDVFCEAWESQISDMSTLLREINDVFEGRRGEKYG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | catenin (cadherin-associated protein), alpha-like 1 |
| 别名 | ACRP, Alpha catenin related protein, alpha CATU, Alpha catulin, Alpha-catenin-related protein |
| 计算分子量 | 82 kDa |
| 观测分子量 | 82 kDa |
| GenBank蛋白编号 | NM_003798 |
| 基因名称 | CTNNAL1 |
| Gene ID (NCBI) | 8727 |
| RRID | AB_3742792 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9UBT7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Catenin alpha-like protein 1 (CTNNAL1, also known as alpha-catulin) is a cytoplasmic molecule that integrates the crosstalk between nuclear factor-kappa B (NF-κB) and Rho signaling pathways. It plays a crucial role in maintaining epithelial integrity and promoting wound repair in bronchial epithelial cells. CTNNAL1 has been shown to inhibit ozone-induced airway epithelial-mesenchymal transition and regulate mucus hypersecretion induced by house dust mite (HDM) (PMID: 38602002). Additionally, CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway (PMID: 36535402). In cancer, CTNNAL1 regulates cancer stem cells (CSCs) in lung cancer and glioblastoma, modulating their migration and invasion abilities (PMID: 37239133).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CTNNAL1 antibody 32926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
