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验证数据展示

  • WB analysis of HeLa using 12599-1-AP

    WB analysis of HeLa using 12599-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human prostate cancer using 12599-1-AP

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 12599-1-AP

    IHC staining of human prostate cancer using 12599-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 12599-1-AP

    IF Staining of HepG2 using 12599-1-AP

    Immunofluorescent analysis of HepG2 cells, using CTBS antibody 12599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CTBS Polyclonal antibody

CTBS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.2.1.-, Di-N-acetylchitobiase, CTB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells, mouse colon tissue
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12599-1-AP targets CTBS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3228

Product name: Recombinant human CTBS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-105 aa of BC024007

Sequence: MSRPQLRRWRLVSSPPSGVPGLALLALLALRLAAGTDCPCPEPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARVVLKGNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chitobiase, di-N-acetyl-
别名 EC:3.2.1.-, Di-N-acetylchitobiase, CTB
计算分子量 12 kDa, 43 kDa
观测分子量 44-50 kDa
GenBank蛋白编号BC024007
基因名称 CTBS
Gene ID (NCBI) 1486
RRIDAB_10638783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for CTBS antibody 12599-1-APDownload protocol
IHC protocol for CTBS antibody 12599-1-APDownload protocol
IF protocol for CTBS antibody 12599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols