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验证数据展示

  • WB analysis of human brain using 12932-1-AP

    WB analysis of human brain using 12932-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12932-1-AP (MARK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12932-1-AP

    IP experiment of mouse brain using 12932-1-AP

    IP result of anti-MARK3 (IP:12932-1-AP, 3ug; Detection:12932-1-AP 1:500) with mouse brain tissue lysate 4000ug.

MARK3 Polyclonal antibody

MARK3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12932-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, PC-3 cells
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12932-1-AP targets MARK3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3805

Product name: Recombinant human CTAK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC024773

Sequence: MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP/microtubule affinity-regulating kinase 3
别名 C TAK1, CTAK1, ELKL motif kinase 2, EMK 2, EMK2, KP78, MARK3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR 1
计算分子量 78 kDa, 80 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC024773
基因名称 MARK3
Gene ID (NCBI) 4140
RRIDAB_10638613
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27448
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MARK3 antibody 12932-1-APDownload protocol
IP protocol for MARK3 antibody 12932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols