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验证数据展示

  • WB analysis of HeLa using 60087-1-Ig

    WB analysis of HeLa using 60087-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60087-1-Ig (CTAGE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 60087-1-Ig

    IF Staining of MCF-7 using 60087-1-Ig

    Immunofluorescent analysis of MCF-7 cells, using CTAGE1 antibody 60087-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • IHC staining of mouse testis using 60087-1-Ig

    IHC staining of mouse testis using 60087-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60087-1-Ig (CTAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTAGE1 Monoclonal antibody

CTAGE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60087-1-Ig

产品说明书

CloneNo. 3B4C2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Cancer/testis antigen 21.2, CT21.1, CT21.2, CT21.2,CTAGE2, CTAGE, CTAGE 1, CTAGE 2, CTAGE1, CTAGE2, Protein cTAGE 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60087-1-Ig targets CTAGE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CTAGE1 fusion protein Ag3948 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cutaneous T-cell lymphoma-associated antigen 1
别名 Cancer/testis antigen 21.2, CT21.1, CT21.2, CT21.2,CTAGE2, CTAGE, CTAGE 1, CTAGE 2, CTAGE1, CTAGE2, Protein cTAGE 2
计算分子量 754 aa, 89 kDa
观测分子量 120 kDa
GenBank蛋白编号BC031065
基因名称 CTAGE1
Gene ID (NCBI) 64693
RRIDAB_2086620
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96RT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CTAGE1 antibody 60087-1-IgDownload protocol
IHC protocol for CTAGE1 antibody 60087-1-IgDownload protocol
IF protocol for CTAGE1 antibody 60087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols