验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human brain tissue, L02 cells, mouse liver tissue | 
| Positive IHC detected in | human brain tissue,  human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
| Positive IF/ICC detected in | HepG2 cells | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 | 
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21143-1-AP targets FLJ23584 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description | 
| 经测试反应性 | human, mouse | 
| 免疫原 | CatNo: Ag15647 Product name: Recombinant human CTA-216E10.6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC007210 Sequence: MNPVPHWGEVFLLVGGEGEHLASQGTTPARDHRVGISPASQQAQPESWRRRQRDKGVDPEKAPSLTRQSQNPPSLTAPLGMPSACSCLPCGPAPEAAIILAGPPTALTVLPKGTGLKKSKRLLLESLMRRRIAHLKWGLPRRILESYFLFNFLGSCSLTLAGARLSGLNTGQELQAQQERYCEAQGSPPGLKSPERFQRVQRPDRKSSKLPIQARALERNRPHMSEPIKHFHPA种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | hypothetical FLJ23584 | 
| 别名 | C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584 | 
| 计算分子量 | 234 aa, 26 kDa | 
| 观测分子量 | 28-30 kDa | 
| GenBank蛋白编号 | BC007210 | 
| 基因名称 | FLJ23584 | 
| Gene ID (NCBI) | 79640 | 
| RRID | AB_10732689 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | C9J442 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FLJ23584 antibody 21143-1-AP | Download protocol | 
| IHC protocol for FLJ23584 antibody 21143-1-AP | Download protocol | 
| WB protocol for FLJ23584 antibody 21143-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 









