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验证数据展示

  • WB analysis of HepG2 using 21143-1-AP

    WB analysis of HepG2 using 21143-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21143-1-AP

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 21143-1-AP

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 21143-1-AP

    IF Staining of HepG2 using 21143-1-AP

    Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FLJ23584 Polyclonal antibody

FLJ23584 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21143-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, L02 cells, mouse liver tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21143-1-AP targets FLJ23584 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FLJ23584 fusion protein Ag15647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypothetical FLJ23584
别名 C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584
计算分子量 234 aa, 26 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC007210
基因名称 FLJ23584
Gene ID (NCBI) 79640
RRIDAB_10732689
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDC9J442
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.

实验方案

Product Specific Protocols
WB protocol for FLJ23584 antibody 21143-1-APDownload protocol
IHC protocol for FLJ23584 antibody 21143-1-APDownload protocol
IF protocol for FLJ23584 antibody 21143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols