验证数据展示

WB analysis using 12245-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12245-1-AP (Cystatin C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 12245-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12245-1-AP (Cystatin C Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of mouse kidney using 12245-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Cystatin C antibody (12245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of Caco-2 using 12245-1-AP
IP result of anti-Cystatin C (IP:12245-1-AP, 4ug; Detection:12245-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.
IF Staining of HeLa using 12245-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cystatin C antibody (12245-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
WB analysis of HepG2 using 12245-1-AP
WB result of Cystatin C antibody (12245-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells.
IHC staining of mouse kidney using 12245-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12245-1-AP (Cystatin C antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 12245-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Cystatin C antibody (12245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Cystatin C Polyclonal antibody

Cystatin C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 12245-1-AP

产品说明书

引用文献(43)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (3)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

CST3, Cystatin 3, Cystatin-3, Cystatin-C, Gamma-trace

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (43)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Caco-2 cells, fetal human brain tissue, HepG2 cells, human milk, HeLa cells, U-87 MG cells, U2OS cells, human urine, human serum, mouse brain tissue
Positive IP detected in	Caco-2 cells
Positive IHC detected in	mouse kidney tissue, human kidney tissue, human ovary tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 36 publications below
IHC	See 3 publications below
IF	See 5 publications below

产品信息

12245-1-AP targets Cystatin C in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, pig, duck
免疫原	CatNo: Ag2890 Product name: Recombinant human Cystatin C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-146 aa of BC013083 Sequence: AGSSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDMYHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQGTMTLSKSTCQDA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cystatin C
别名	CST3, Cystatin 3, Cystatin-3, Cystatin-C, Gamma-trace
计算分子量	146 aa, 16 kDa
观测分子量	13 kDa
GenBank蛋白编号	BC013083
基因名称	Cystatin C
Gene ID (NCBI)	1471
RRID	AB_2088058
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P01034
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

实验方案

Product Specific Protocols
WB protocol for Cystatin C antibody 12245-1-AP	Download protocol
IP protocol for Cystatin C antibody 12245-1-AP	Download protocol
IHC protocol for Cystatin C antibody 12245-1-AP	Download protocol
IF protocol for Cystatin C antibody 12245-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. Authors - Xinyan Li View Article
mouse	WB	Exp Mol Med YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis Authors - Wei Su View Article
mouse	IF	Neuron Macrophage-derived CTSS drives the age-dependent disruption of the blood-CSF barrier Authors - Yifan Chen View Article
human	WB,IF	Cell Death Differ Transcriptional and ubiquitinative suppression of macrophage CST3 disrupts colonic homeostasis through defective efferocytosis. Authors - Honggang Wang View Article
pig	WB	Biosens Bioelectron Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic. Authors - Yi-Jyun Chen View Article
human	WB	J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. Authors - Feng Xiaoli View Article

验证数据展示

WB analysis using 12245-1-AP

IHC staining of human kidney using 12245-1-AP

IF Staining of mouse kidney using 12245-1-AP

IP experiment of Caco-2 using 12245-1-AP

IF Staining of HeLa using 12245-1-AP

WB analysis of HepG2 using 12245-1-AP

IHC staining of mouse kidney using 12245-1-AP

IF Staining of mouse kidney using 12245-1-AP

Cystatin C Polyclonal antibody

Cystatin C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2.

YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis

Macrophage-derived CTSS drives the age-dependent disruption of the blood-CSF barrier

Transcriptional and ubiquitinative suppression of macrophage CST3 disrupts colonic homeostasis through defective efferocytosis.

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

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