验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10892-2-AP targets CSRP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CSRP2 fusion protein Ag1338 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cysteine and glycine-rich protein 2 |
| 别名 | CRP2, CSRP2, Cysteine rich protein 2, LIM domain only protein 5, LMO 5, LMO5, SmLIM, Smooth muscle cell LIM protein |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC000992 |
| 基因名称 | CSRP2 |
| Gene ID (NCBI) | 1466 |
| RRID | AB_2087738 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16527 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSRP2 antibody 10892-2-AP | Download protocol |
| IP protocol for CSRP2 antibody 10892-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells. | ||
Toxicol Appl Pharmacol Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif
| ||
Cell Biochem Funct Cysteine and glycine-rich protein 2 retards platelet-derived growth factor-BB-evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity
| ||
Toxicol Appl Pharmacol Cysteine and glycine-rich protein 2 is crucial for maintaining the malignant phenotypes of gliomas through its action on Notch signalling cascade | ||
Oncol Rep Cysteine‑ and glycine‑rich protein 2: A vital regulator that inhibits necroptosis glioma cell by activating the JAK‑STAT1 pathways
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