验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31623-1-AP targets CSPG4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chondroitin sulfate proteoglycan 4 |
| 别名 | NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP |
| 计算分子量 | 251kd |
| 观测分子量 | 250-300 kDa |
| GenBank蛋白编号 | NM_001897.5 |
| 基因名称 | CSPG4 |
| Gene ID (NCBI) | 1464 |
| RRID | AB_3670052 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6UVK1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSPG4 is a proteoglycan that plays a role in cell proliferation and migration, stimulates endothelial cell motility during microvascular morphogenesis and may also inhibit neurite outgrowth and growth cone collapse during axon regeneration. CSPG4 is expressed on more than 90% of human melanoma tissues and cultured cells. It is a target of specific diagnosis and immunotherapy with antiidiotypic antibodies due, in part, to its roles in growth control, adhesion, cell-substratum interactions, and cell-cell contacts (PMID: 10587647; 11278606).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSPG4 antibody 31623-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
