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验证数据展示

  • WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

    WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 68200-1-Ig (same clone as 68200-1-PBS)

    WB analysis of A549 using 68200-1-Ig (same clone as 68200-1-PBS)

    WB result of CSNK2A1 antibody (68200-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

    IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CSNK2A1 Monoclonal antibody, PBS Only

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68200-1-PBS

产品说明书

CloneNo. 1D5E8

宿主/亚型

Mouse / IgG1

种属反应性

human, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

1D5E8, CK II alpha, CK2a, CK2A1, CK2α

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CSNK2A1 Ready-To-Use IHC Kit. CSNK2A1 primary antibody included.

产品信息

68200-1-PBS targets CSNK2A1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rabbit
免疫原

CatNo: Ag29062

Product name: Recombinant human CSNK2A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-391 aa of BC011668

Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 casein kinase 2, alpha 1 polypeptide
别名 1D5E8, CK II alpha, CK2a, CK2A1, CK2α
计算分子量 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC011668
基因名称 CSNK2A1
Gene ID (NCBI) 1457
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP68400
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

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