验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy CSNK1A1 Ready-To-Use IHC Kit. CSNK1A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68434-1-Ig targets CSNK1A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | casein kinase 1, alpha 1 |
| 别名 | CSNK, CKI-alpha, CKI alpha, CK1α, CK1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | NM_001892 |
| 基因名称 | CSNK1A1 |
| Gene ID (NCBI) | 1452 |
| RRID | AB_3073926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P48729 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK1A1 antibody 68434-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
