验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, RAW264.7 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17420-1-AP targets CSGALNACT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11436 Product name: Recombinant human CSGALNACT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 195-542 aa of BC030268 Sequence: DEDDEQEDEEGPLGEKLIFNENDFVEGYYRTERDKGTQYELFFKKADLTEYRHVTLFRPFGPLMKVKSEMIDITRSIINIIVPLAERTEAFVQFMQNFRDVCIHQDKKIHLTVVYFGKEGLSKVKSILESVTSESNFHNYTLVSLNEEFNRGRGLNVGARAWDKGEVLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFYPVVFSLYNPAIVYANQEVPPPVEQQLVHKKDSGFWRDFGFGMTCQYRSDFLTIGGFDMEVKGWGGEDVHLYRKYLHGDLIVIRTPVPGLFHLWHEKRCADELTPEQYRMCIQSKAMNEASHSHLGMLVFREEIETHLHKQAYRTNSEAVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chondroitin sulfate N-acetylgalactosaminyltransferase 2 |
| 别名 | Beta4GalNAcT 2, Beta4GalNAcT-2, CHGN2, Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2, Chondroitin sulfate N-acetylgalactosaminyltransferase 2 |
| 计算分子量 | 542 aa, 63 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC030268 |
| 基因名称 | CSGALNACT2 |
| Gene ID (NCBI) | 55454 |
| RRID | AB_2083878 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N6G5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSGALNACT2 (Chondroitin Sulfate N-Acetylgalactosaminyltransferase 2) is a glycosyltransferase localized to the Golgi membrane that catalyzes the transfer of N-acetylgalactosamine (GalNAc) from the donor substrate UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA) on growing glycosaminoglycan chains. Increased expression of CSGALNACT2 has been observed in the arterial wall during the progression of atherosclerosis, contributing to the elongation of CS chains that may promote lipid retention and smooth muscle cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSGALNACT2 antibody 17420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
