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验证数据展示

  • FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control. Cells were then stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), and 0.25 ug APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1

    Sandwich ELISA standard curve of MP00953-1, Human CSF2RA/CD116 Recombinant Matched Antibody Pair - PBS only. 98066-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02335. 84023-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    The mean CSF2RA/CD116 concentration was determined to be 84.1 ng/mL in THP-1 cell extract based on a 4.0 mg/mL extract load and 59.0 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

  • Sample test of MP00953-1

    Sample test of MP00953-1

    Serum of six individual healthy human donors was measured. The CSF2RA/CD116 concentration of detected samples was determined to be 2.0 ng/mL with a range of 0.9-4.7 ng/mL.

  • Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1

    Cytometric bead array standard curve of MP00953-1, CSF2RA/CD116 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98066-1-PBS. Detection antibody: 84023-1-PBS. Standard: RP02335. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 98066-1-RR

    Affinity and Kinetic Characterization of 98066-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98066-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.52 nM.

Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody, PBS Only

CSF2RA/CD116 Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98066-1-PBS

产品说明书

CloneNo. 241266B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98066-1-PBS targets CSF2RA/CD116 in FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with human samples.

经测试应用 FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
别名 CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA
计算分子量 46 kDa
GenBank蛋白编号BC002635
基因名称 CD116
Gene ID (NCBI) 1438
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP15509
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.