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验证数据展示

  • WB analysis of THP-1 using 18307-1-AP

    WB analysis of THP-1 using 18307-1-AP

    WB result of CSF2RA/CD116 antibody (18307-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSF2RA/CD116 transfected THP-1 cells.

  • WB analysis using 18307-1-AP

    WB analysis using 18307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18307-1-AP

    IHC staining of human placenta using 18307-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 18307-1-AP

    IHC staining of human placenta using 18307-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CSF2RA/CD116 Polyclonal antibody

CSF2RA/CD116 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18307-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD116, GMCSFR alpha, GMCSFR, GM CSF R alpha, CSF2RA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18307-1-AP targets CSF2RA/CD116 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
别名 CD116, GMCSFR alpha, GMCSFR, GM CSF R alpha, CSF2RA
计算分子量 47 kDa
观测分子量55 kDa
GenBank蛋白编号BC071835
基因名称 CD116
Gene ID (NCBI) 1438
RRIDAB_2229990
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

实验方案

Product Specific Protocols
WB protocol for CSF2RA/CD116 antibody 18307-1-APDownload protocol
IHC protocol for CSF2RA/CD116 antibody 18307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols