验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34701-1-AP targets CSF1R/CD115 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | colony stimulating factor 1 receptor |
| 别名 | CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor |
| 计算分子量 | 972 aa, 108 kDa |
| 观测分子量 | 140, 160 kDa |
| GenBank蛋白编号 | BC047521 |
| 基因名称 | CSF1R/CD115 |
| Gene ID (NCBI) | 1436 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P07333 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSF1R (colony-stimulating factor 1 receptor), also known as CD115, is a receptor tyrosine kinase of the class III receptor tyrosine kinase family that regulates the development and function of the mononuclear phagocyte system. It is primarily expressed on monocytes, macrophages, microglia, and their progenitors. CSF1R is activated by its ligands colony-stimulating factor 1 (CSF1/M-CSF) and interleukin-34 (IL-34), leading to receptor dimerization and activation of downstream signaling pathways, including PI3K/AKT, MAPK/ERK, JAK/STAT, and SRC family kinases. Through these pathways, CSF1R regulates cell survival, proliferation, differentiation, migration, and phagocytic activity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSF1R/CD115 antibody 34701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
